peptide mass fingerprinting (http://www.matrixscience.com/cgi/search_form.pl?FORMVER=2&SEARCH=PMF), a search engine that employs MS data to identify proteinsfrom the NCBInr database. The taxonomy for these searcheswas limited to Rattusnorvegicus. The database allowed up to one missed cleavage, and the mass tolerancewas set as 75 ppm peptide limited by fixed modification of carbamidomethyland variable modification of oxidation with monoisotopic values. Mascot reportedthe molecular weight search (MOWSE) score, which is calculated by −10 × log10 (P),where P is the probability that the observed match is a random event. The p valueis limited by the size of the sequence database being searched (limited by taxonomy),the conditions, and the settings of the trypsin digestion. Each calculated valuethat falls within a given mass tolerance of an experimental value counts as a match.The accepted threshold is that an event is significant when it would be expectedto occur at random with a frequency of <5%. In this study, a protein match with ascore of >61 was regarded as significant
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