Fig. 1. Effect of 90% (v/v) ethanol on the temperature of aqueous medium (initial temperature = 0.4 C) in only one stage (A) and in three stages (B): Temperature before (j)
and after (h) adding ethanol. Effect of ethanol concentration on bromelain activity (C). SDS/PAGE analysis and native-PAGE of bromelain using a 14% (w/v) polyacrylamide
gel: lane S, molecular mass standards (in kDa); lane 1, commercial bromelain (10 lg); lane 2, purified bromelain (5 lg); lane 3, crude extract (10 lg); lane 4, native-PAGE of
the purified, cryoprotected bromelain (5 lg) (D).