A Simple, accurate and rapid RP-HPL

A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of doxycycline (DOXY) in bulk and pharmaceutical dosage forms using an Altima C 18, 150 x 4.6 mm i.d, 5 µm particle size in isocratic mode; with mobile phase comprising of buffer (0.1% of potassium dihydrogen phosphate) and acetonitrile in the ratio 60:40 (v/v). The flow rate was 1 ml/min and detection was carried out by photodiode array detector at 268 nm. The retention time for DOXY was found to be 2-897 min. The proposed method has permitted the quantification of DOXY over linearity in the range of 25 – 150
µg/ml and its percentage recovery was found to be 99.134 – 101.997 %. The % RSD of intraday and inter day precision were found 0.7999% and 1.3%.

ผลลัพธ์ (ลาว) 1:

ວິ​ທີ​ການ RP HPLC​, A ງ່າຍ​ດາຍ​, ຖືກ​ຕ້ອງ​ແລະ​ຢ່າງ​ວ່ອງ​ໄວ​ໄດ້​ຮັບ​ການ​ພັດ​ທະ​ນາ​ສໍາ​ລັບ​ການ​ປະ​ມານ​ຂອງ​ດົກ​ຊີ​ຊີກ​ລິນ (Doxy​) ໃນ​ຈໍາ​ນວນ​ຫລາຍ​ແລະ​ຢາ​ແບບ​ຢາ​ໃຊ້ Altima C 18​, id 150 x 46 ມ​ມ​, 5 m ຂະ​ຫນາດ particle ໃນ​ຮູບ​ແບບ isocratic​; ກັບ​ໂຄງ​ການ​ໄລ​ຍະ​ໂທລະ​ສັບ​ມື​ຖື​ປະ​ກອບ​ດ້ວຍ​ກັນ (01​% ຂອງ​ຟ​ອ​ສ​ເຟດ dihydrogen potassium​) ແລະ acetonitrile ໃນ​ອັດ​ຕາ​ສ່ວນ 60:40 (v / v​)​. ອັດ​ຕາ​ການ​ໄຫຼ​ແມ່ນ 1 ml / ນາ​ທີ​ແລະ​ການ​ຊອກ​ຄົ້ນ​ຫາ​ໄດ້​ດໍາ​ເນີນ​ການ​ໂດຍ​ເຄື່ອງ​ກວດ​ຈັບ​ອາ photodiode ທີ່ 268 nm​. ທີ່​ໃຊ້​ເວ​ລາ​ການ​ຮັກ​ສາ​ໄວ້​ສໍາ​ລັບ​ການ Doxy ໄດ້​ພົບ​ເຫັນ​ວ່າ​ເປັນ 2-897 min​. ວິ​ທີ​ການ​ນໍາ​ສະ​ເຫນີ​ໄດ້​ອະ​ນຸ​ຍາດ​ປະ​ລິ​ມານ​ຂອງ Doxy ໃນ​ໄລ​ຍະ​ແຂບ​ຢູ່​ໃນ​ລະ​ດັບ​ຂອງ 25 - 150
g / ml ແລະ​ການ​ຟື້ນ​ຕົວ​ຂອງ​ອັດ​ຕາ​ສ່ວນ​ຂອງ​ຕົນ​ໄດ້​ພົບ​ເຫັນ​ວ່າ​ເປັນ 99,134 - 101,997​%​. % RSD ຂອງ​ການ​ຈະ​ວັນ intraday ແລະ​ລະ​ຫວ່າງ​ທີ່​ໄດ້​ພົບ​ເຫັນ 0,7999​% ແລະ 13​%​.