animals were sacrificed by over
dosage of ether anesthesia. The wound biopsies
were collected and stored at -800C until analysis.
Difficulty was encountered in collecting the fourth
day wounds thus fourth day sampling was not
done. The granulation tissue was subjected for
estimation of biochemical parameters. As an
indication of total collagen content,
hydroxyproline concentration was determined as
described by Woessner. To estimate
hexosamine the tissue samples were defatted in
chloroform:methanol (2:1) and dried in acetone,
before use. Hexosamine was estimated by the
method of Elson and Morgon respectively.
Lipid peroxide levels in granulation tissues were
determined by the thiobarbituric acid reaction