The primer set suggested in this study is appropriate for screening for the four viruses in clinical specimens from infants and children with acute gastroenteritis in Asia, Europe, and America. However, it can be modified according to the situation in the target countries. In this study, Aichi virus was not found in clinical specimens. In our previous studies, as well as in other studies conducted in Asian, European, and American countries, low detection rates of Aichi virus were reported. However, Aichi virus was found in higher frequencies in African countries, such as Tunisia (Sdiri-Loulizi et al.,
2008). Its detection rate was found to be closer to, or even higher than, those of astrovirus, adenovirus, and sapovirus. In such a situation, the primer pair to detect Aichi virus can be excluded from the list and added to the common virus primer list.