(i)
(ii)
(iii) (iv)
(v)
DATA is a gene expression data matrix with samples in columns. The first row contains the information of the experimental condition of each sample. The remaining rows contain gene expression.
GS is a binary matrix coded 0 or 1 with genes in rows. Each column represents a predefined gene set, with row equal to 1 indicating that the gene is in the gene set, and 0 otherwise.
Alpha is the significance level.
nbPerm specifies the number of permutations, and at
least 5,000 is recommended.
MCP specifies one of three multiple comparison methods, Dunnett = 1, Tukey = 2, and sequential pairwise comparison = 3. MCP can be ignored when the number of experimental conditions is 2.