DISCUSSION and CONCLUSION
Glycine max., which is naturally diploid, due to its vegetative characteristics, is used both as a feed crop and an industrial crop [10]. Tissue culture studies conducted
on Glycine max. are limited in number with mostly in vitro propagation having been performed [2]. In the present study, in order to obtain explants, seeds were germinated under sterile conditions so that plantlets would be regenerated. Following the sterilization of their surface, seeds were ground or rubbed without any further procedure, and were left to germinate in hormone-free MS medium and on filter paper [5]. No problem was encountered during the germination stage, and seeds were
inoculated into hormone-free MS medium leading to the formation of regenerated plantlets, ready for obtaining explants, within a period of 21 days. The findings of the present study demonstrated that the optimization of the tissue culture conditions of soybean, which is a short-day plant and of which the yield and quality are influenced by environmental factors, may contribute greatly to its
cultivation as a main crop in Konya province and the reorganization of the production pattern of the region [4]. By means of the tissue culture of seed explants, a new cultivar specific to the species and origin investigated, was obtained for soybean, which is influenced readily by environmental conditions [7]. The MS medium yielded a satisfactory reproduction rate with respect to the viability
and number of sprouts under tissue culture conditions. Accordingly, it was concluded that, a satisfactory reproduction yield was obtained from the tissue culture method in the present study, which was aimed at the propagation and culture of soybean. A rooting rate of 80% was achieved with the indicated method. The developed stage of the soybean crop following tissue culture under
in vitro conditions is presented in Figure 5.
DISCUSSION and CONCLUSIONGlycine max., which is naturally diploid, due to its vegetative characteristics, is used both as a feed crop and an industrial crop [10]. Tissue culture studies conductedon Glycine max. are limited in number with mostly in vitro propagation having been performed [2]. In the present study, in order to obtain explants, seeds were germinated under sterile conditions so that plantlets would be regenerated. Following the sterilization of their surface, seeds were ground or rubbed without any further procedure, and were left to germinate in hormone-free MS medium and on filter paper [5]. No problem was encountered during the germination stage, and seeds wereinoculated into hormone-free MS medium leading to the formation of regenerated plantlets, ready for obtaining explants, within a period of 21 days. The findings of the present study demonstrated that the optimization of the tissue culture conditions of soybean, which is a short-day plant and of which the yield and quality are influenced by environmental factors, may contribute greatly to itscultivation as a main crop in Konya province and the reorganization of the production pattern of the region [4]. By means of the tissue culture of seed explants, a new cultivar specific to the species and origin investigated, was obtained for soybean, which is influenced readily by environmental conditions [7]. The MS medium yielded a satisfactory reproduction rate with respect to the viabilityand number of sprouts under tissue culture conditions. Accordingly, it was concluded that, a satisfactory reproduction yield was obtained from the tissue culture method in the present study, which was aimed at the propagation and culture of soybean. A rooting rate of 80% was achieved with the indicated method. The developed stage of the soybean crop following tissue culture underin vitro conditions is presented in Figure 5.
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