G. hansenii strain was used in this study. This strain has been
isolated from inoculums of a strain screened in homemade vinegar
and then induced by high hydrostatic pressure treatment. It has a
strong ability to produce BC more than three times as that of its
initial strain. It was deposited as CGMCC3917 at China General
Microbiological Culture Collection, Beijing, China and was maintained
on glucose agar slants containing: 2% glucose (w/v), 0.5%
yeast extract (w/v), 0.1% K2HPO4 (w/v), 1.5% MgSO47H2O (w/v),
1.5% ethanol (v/v), and 1.7% agar (w/v). It was stored at 4 C in a
refrigerator and sub-cultured every 2 months for inoculum development
or stored at 80 C using 20% (v/v) glyc