Fig. 1. Ethidium bromide-stained agarose gel of polymerase chain reaction amplicons
confirming the deletion of the hrcC gene in Acidovorax citrulli strain 00-1. Lane 1, 1-kb
DNA ladder (Fermentas, Glen Burnie, MD); lane 2, AAC00-1ΔhrcC (nonpathogenic
hrcC mutant of AAC00-1); lane 3, AAC00-1ΔhrcCcomp (complemented hrcC mutant
of AAC00-1); lane 4, AAC00-1 (wild-type A. citrulli); lane 5, water (negative control).