The five treatment regimes were duplicated at 10 and 20°C, with 10 replicates of each. Each replicate consisted of one adult female in a jar containing 20 ml of the treatment solution. The animals were checked every 12 h, when deaths were recorded and used to calculate percentage survival over time. Animals were transferred to fresh medium in clean jars every 48 h and fed 1.5 mg l–1 dry weight Cryptomonas. This amount was less than in Experiment 1 because only one animal was being sustained; however, it was still above the incipient limiting amount for female B. hamata (Burns and Hegarty, 1994). The times until death were calculated from (i) the start of the treatment regime, (ii) transfer to any salinity higher than 500 mg l–1 Cl and (iii) transfer to the highest salinity of 1500 mg l–1 Cl. The experiment ran for 25 days, by which time most animals had died. The results were analysed by two-way ANOVA (DataDesk 4.1), using temperature and treatment (acclimation pattern) as the variables. Scheffé post hoc tests were conducted to determine individual significant differences.