In this work, an amino (NH2) column was used to separate melamine in dairy products, acetonitrile was used here as both of
mobile phase component and protein precipitant. Factors of affecting retention of melamine were explored, including buffer
concentration, pH, and percentage of organic solvent in the mobile phase. The optimal UV detection wavelength was selected. Satisfactory retention of melamine, good peak shape, and high sensitivity were obtained under the chosen conditions