Fig. 6. Methylene blue analysis the aleurone layers in mature seeds of WT and OsLTPL36-RNAi lines. (A) Mature seeds of WT and (B, C and D) OsLTPL36-RNAi lines (i5-5, i8-3 and i23-2) were stained with methylene blue and then analyzed by light microscopy. The mature seeds of WT (A) and three RNAi lines (B–D). Down panel is the enlarged view of seed dorsal side. Bars: 0.5 mm in (A–D); 0.2 mm in (E–H).