3. Reagents
a. ammonia-free water: See Saction 4500-NH .B.3a. Use for making all regents
b. Sodium hydroxide,10N.
c. NaoH/EDTA solution.10N:Dissolve 400 g NaOH in 800 ml water Add 45.2 g ethylenediaminetetraacetic acid,tetrasodium salt, tetrahydrate () and stir to dissolve. Cool and dilute to 1000 ml