I want Sarawut to carry out a number of things with this data and write a single report that includes all information in appendices:
1) We need an overview in a spreadsheet that links the file names from the PGM to the actual samples that Baset provided. This overview should be clear and intuitive. Please include providence information for each sample (Baset can provide this).
2) A number of variables have influenced the quality and reads per sample: DNA extraction, PCR amplification success, chip loading and running. Sarawut should have all this information available and I want to have a report that interprets the data. Please include aspects such as: visual sample quality, Qubit DNA measurements, Fragment analyser measurements, the PGM run reports provided by Audun, and the quality graphs provided here. We need to know what variables influence the output, i.e. should be invest time and money in an extract with little DNA, no amplification, etc, or does it not matter and is the primer fit (ITS1 or ITS2) of greater importance.
3) Per sample we need to know what species we found in the DNA mixture. To what species were our MOTUs (the clusters) identified by BLAST. How many reads do we have per species in each cluster. How different are the results for ITS1 and ITS2 for the same sample. Consider using graphic visualisation for this: http://www.datavizcatalogue.com/index.html. Make sure to interpret the results from tables and figures in text as well.
# This is a lot of work and I want it to be carried out to meticulous standard. Please be sure to communicate any questions with us in order to stay on the right track. We are very happy to help you interpret this data, because we want to see the results and we will all learn a lot in the process. So feel free to ask us anything at anytime. I want you to present these results at the Drøbak meeting at the end of November.
All the best for now,
I want Sarawut to carry out a number of things with this data and write a single report that includes all information in appendices:1) We need an overview in a spreadsheet that links the file names from the PGM to the actual samples that Baset provided. This overview should be clear and intuitive. Please include providence information for each sample (Baset can provide this).2) A number of variables have influenced the quality and reads per sample: DNA extraction, PCR amplification success, chip loading and running. Sarawut should have all this information available and I want to have a report that interprets the data. Please include aspects such as: visual sample quality, Qubit DNA measurements, Fragment analyser measurements, the PGM run reports provided by Audun, and the quality graphs provided here. We need to know what variables influence the output, i.e. should be invest time and money in an extract with little DNA, no amplification, etc, or does it not matter and is the primer fit (ITS1 or ITS2) of greater importance.3) Per sample we need to know what species we found in the DNA mixture. To what species were our MOTUs (the clusters) identified by BLAST. How many reads do we have per species in each cluster. How different are the results for ITS1 and ITS2 for the same sample. Consider using graphic visualisation for this: http://www.datavizcatalogue.com/index.html. Make sure to interpret the results from tables and figures in text as well.# เป็นการทำงานมาก และฉันต้องการดำเนินการมาตรฐานที่พิถีพิถัน กรุณาให้แน่ใจว่าได้ติดต่อสอบถามกับเราจึงมุ่ง เรามีความสุขมากเพื่อช่วยในการแปลข้อมูลนี้ เนื่องจากเราต้องการดูผลลัพธ์ และเราจะทั้งหมดได้เรียนรู้มากในกระบวนการ ความรู้สึกเพื่อสอบถามเราอย่างเต็มที่ ฉันต้องการคุณสามารถนำเสนอผลลัพธ์เหล่านี้ในการประชุม Drøbak เมื่อสิ้นสุดเดือนพฤศจิกายนทั้งหมดดีที่สุดสำหรับตอนนี้
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