Bicarbonate alkalinity and carbon can be provided by the addition of sodium bicarbonate to the cultivation medium.
In synthetic cultivation media for the cultivation of Spirulina sp. the most frequently used medium is Zarrouk medium,which provides carbon as sodium bicarbonate in the amount of 16.8 g L1 . In the earlier studies, sodium bicarbonate was added to media containing anaerobically digested wastes
in amounts in the range of 9–17 g L1 , but 2–4 g L1 is now considered as sufficient . Furthermore, suggested that supplementation of sodium bicarbonate need not exceed 0.5% of the culture medium to be adequate for Spirulina growth in effluents of anaerobically digested pig and cattle manure. It has been suggested that many factors, including
bicarbonate (HCO3) furnishes the carbon skeleton needed for algal biomass particularly, proteins. It may be essential for protein synthesis in the organism, probably because sodium is necessary for the activity of certain enzymes involved
in protein synthesis, formation of coenzymes and adenosine phosphates and biosynthesis of sulfur-containing proteins, respectively . Moreover, algae put up different metabolic products during photosynthesis, using only light and nutrients. The relative amounts of these products are tightly linked to environmental and nutrient conditions, including CO2 levels. Carbon is required as non-mineral nutrient for algal growth as as reported by Walker. Similar to the results reported from this study, the OD680 and biochemical constituents of the tested alga had greatly affected when grown under different
concentrations of sodium bicarbonate as compared by control culture after 12 days. The statistical analyses have already
revealed that sodium bicarbonate exerted a significant positive effect on the algal constituents of proteins and chlorophyll
(p > 0.01). In accordance with the study results, previous studies have shown sodium bicarbonate can be added as a form of inorganic carbon to increase cell dry weight, FAME, and pigment production . Even though, reported that inorganic carbon of alkalinity in the form of bicarbonate was consumed rapidly, in turn causing the attenuation of cell
growth of Scenedesmus sp. Moreover, it has been reported that the elevated CO2 concentrations decrease the relative concentrations of proteins and pigments in the cells but increase carbohydrate content of the A. platensis which was accompanied by reduction in the maximum biomass yield as reported by Gordillo et al.
Bicarbonate alkalinity and carbon can be provided by the addition of sodium bicarbonate to the cultivation medium.In synthetic cultivation media for the cultivation of Spirulina sp. the most frequently used medium is Zarrouk medium,which provides carbon as sodium bicarbonate in the amount of 16.8 g L1 . In the earlier studies, sodium bicarbonate was added to media containing anaerobically digested wastesin amounts in the range of 9–17 g L1 , but 2–4 g L1 is now considered as sufficient . Furthermore, suggested that supplementation of sodium bicarbonate need not exceed 0.5% of the culture medium to be adequate for Spirulina growth in effluents of anaerobically digested pig and cattle manure. It has been suggested that many factors, includingbicarbonate (HCO3) furnishes the carbon skeleton needed for algal biomass particularly, proteins. It may be essential for protein synthesis in the organism, probably because sodium is necessary for the activity of certain enzymes involvedin protein synthesis, formation of coenzymes and adenosine phosphates and biosynthesis of sulfur-containing proteins, respectively . Moreover, algae put up different metabolic products during photosynthesis, using only light and nutrients. The relative amounts of these products are tightly linked to environmental and nutrient conditions, including CO2 levels. Carbon is required as non-mineral nutrient for algal growth as as reported by Walker. Similar to the results reported from this study, the OD680 and biochemical constituents of the tested alga had greatly affected when grown under differentconcentrations of sodium bicarbonate as compared by control culture after 12 days. The statistical analyses have already
revealed that sodium bicarbonate exerted a significant positive effect on the algal constituents of proteins and chlorophyll
(p > 0.01). In accordance with the study results, previous studies have shown sodium bicarbonate can be added as a form of inorganic carbon to increase cell dry weight, FAME, and pigment production . Even though, reported that inorganic carbon of alkalinity in the form of bicarbonate was consumed rapidly, in turn causing the attenuation of cell
growth of Scenedesmus sp. Moreover, it has been reported that the elevated CO2 concentrations decrease the relative concentrations of proteins and pigments in the cells but increase carbohydrate content of the A. platensis which was accompanied by reduction in the maximum biomass yield as reported by Gordillo et al.
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