The optimization of operating
factors resulted in high lignin removal efficiency equal to 74% in a 30 day pretreatment. The maximum
enzymatic saccharification of pretreated biomass at optimum conditions was obtained equal to 56%.
Conversion of cellulose to reducing sugars during the pretreatment stage was 21% which suggests that
higher saccharification efficiency could be achieved in a simultaneous pretreatment and saccharification
process.