3.3. Characterisation of the present HPLC method for honey adulteration detection
As discussed above, the adulterated honey samples with syrup can be detected according to the presence of this characteristic peak of syrup at 15.25 min retention time. Here, this method was characterised on linearity. A series fraud honey samples were pre- pared in laboratory by intermingling one authentic acacia honey and one rice HFS sample in different mass proportions, 2.5–100%. HPLC chromatograms of these artificial honey samples were shown by overlaying signals in Fig. 5(a) and (b). Along with the increasing