The microplate was settled at room temperature for 15min and the sample fluorescence was then scanned again (Second scan). The RNA concentrations (μg mL−1) were calculated as the differences between first (DNA + RNA) and second (DNA) scans, and the standard curve of RNA versus the fluorescence established from the first scan of the same plate. DNA concentrations (μg mL−1) were computed from the second scan and the standard curve of DNA versus fluorescence established at the second scan (RNase treated) of the same plate.