B: base consumption in ml (or L), Nb: normality of the base,
˛: average degree of dissociation of the -NH groups, PM:mass
of protein in g (or kg), htot.: total number of peptide bonds in
meqv/g protein, and HD: hydrolysis degree %.
˛−1 is the calibration factor for the pH-stat, and it is the
reciprocal of the degree of dissociation