1 g of defatted flowers or leaves were taken and vessel shifted into filter paper thimble.160 ml of aqueous ethanol were poured into round bottom flask (250 ml capacity)followed by fitting it on soxhlet assembly on water bath.Extraction time 5 h,until the clear solution was observed in Soxhlet,where thimble was placed.Solvent was then evaporated under vacuum and the extract was diluted with same solvent and volume was made 50 ml in volumetric flask.