2.3.6. Diastase activity
Honey diastase activity was determined according to AOAC
method 958.09, by placing 5 g of honey into a 20 ml beaker and
diluting with 10 ml distilled water and 2.5 ml of acetate buffer
(1.59 M, pH 5.3). It was transferred to a 25 ml volumetric flask containing
1.5 ml of 0.5 M NaCl solution. Ten ml of honey solution
were incubated in a thermostatic bath at 40 C along with a second
flask containing 100 ml of 1% (w/v) starch solution. After 5 min,