Adult hoppers are removed from the oviposition cage and the nymphs are allowed to emerge. Each rearing cage can accommodate six to eight potted plants that can sustain 1000-1400 hoppers. The standard test insects(1-to 2-day-old female adults) are collected from these daily rearing cages. The plants are replaced every 2 days (or as needed) and transferred to new rearing cages(labeled with egg collection dates and locations)