The single spot comprising the purified biosurfactant was scrapped off from the TLC plate and then evaluated for its surfactant activity by oil spreading test. Such TLC-purified biosurfactant was utilized for further analysis.
The single spot comprisingthe purified biosurfactant was scrapped off from the TLCplate and then evaluated for its surfactant activity by oil spreadingtest. Such TLC-purified biosurfactant was utilized for furtheranalysis.