At 24 hrs before the test, an area of skin approximately 5 cm × 5 cm on the dorso-lumbar region of each rat was clipped free of hairs. Care was taken to avoid abrading on the skin. The
PM at the dose of 2,000 mg/kg body weight was introduced onto the 3.5 cm × 3.5 cm gauze patch, applied on the shaven skin of the rats of the treatment group. In the same manner, base cream at equivolume to the treatment group was used in place of the test sample and applied to the rat’s skin of the control group. The patches were then secured onto the skin by transpore