The need of MS salts for shoot sprouting and proliferation shows the high salt requirement for the growth of Gymnema sylvestre. Influence of plant growth regulators, IAA, BA, 2,4-D and kinetin on shoot sprouting was investigated. Synergistic effect of Vitamin B2 was studied. To overcome phenolic exudation and the effects of antioxidants, the effect of activated charcoal, citric acid and ascorbic acid were investigated. Protocol for rapid in vitro propagation of Ceropegia candelabrum L. (Asclepiadaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 8.87 μM N 6-benzyladenine (BA) and 2.46 μM indole-3-butyric acid (IBA) was best Schematic representation of large scale in vitro plantlet production from mature axillary node explants of Gymnema sylvestre.