Our results demonstrate that a bout of morning resistance training targeting both upper and lower body musculature can potentiate performance in maximal sprints, lifting and jumping later in the day. This long-term potentiation may offer some advantage to sports involving a broad base of power application. In addition, morning exercise attenuated the diurnal fall in free testosterone, suggestive of a hormone-mediated effect on subsequent performance (potentially via improved motivation). In addition we found that morning sprints potentiated afternoon sprints only, which suggests a movement-specific potentiating effect.