However, YFAME gradually decreases with high nM/nO ratios, specifically when the nM/nO ratio is above 6. One interpretation of these data is that a nM/nO ratio higher than 6 slightly influences the chemical equilibrium (Colucci et al., 2005; Noureddini et al., 1998; Ma et al., 1999; Meher et al.,2006a).