The uterus of control animals showed implantation sites (Figure 2(a)). No implantation sites were observed in the uterus from treatment group animals (n-hexane fraction of V. negundo leaf extract) (Figure 2(b)). Similarly, Figure 3 depicts graph showing total number of implants in uterus of control mice and mice treated with n-hexane fraction, chloroform fraction, n-butanol fraction, and remnant fraction of Vitex negundo Linn leaf extract treated mice, respectively. Even though animals treated with n-butanol, chloroform, and remnant fraction of Vitex negundo leaf extract showed partial inhibition in blastocyst implantation, inhibition is statistically significant when compared to control animals. No implantation site has been observed in mice treated with n-hexane fraction of V. negundo leaf extract (Figure 3).