Enological strains grown
overnight in 10 ml MRS broth at 30 C were pelleted by centrifugation,
washed twice with phosphate buffer (0.1 M, pH 7.0), and
resuspended in 2 ml of Ringer solution (8.5 g/L NaCl, 0.4 g/L KCl,
0.34 g/L hydrated CaCl2, SigmaeAldrich).
Enological strains grownovernight in 10 ml MRS broth at 30 C were pelleted by centrifugation,washed twice with phosphate buffer (0.1 M, pH 7.0), andresuspended in 2 ml of Ringer solution (8.5 g/L NaCl, 0.4 g/L KCl,0.34 g/L hydrated CaCl2, SigmaeAldrich).
การแปล กรุณารอสักครู่..
![](//thimg.ilovetranslation.com/pic/loading_3.gif?v=b9814dd30c1d7c59_8619)