To be considered as positive, a signal
generated in the CoSYPS Path Food detection system should display an
(exponential) amplification above the limit of detection of each qPCR
determined previously, with the expected Tm-value (Barbau-Piednoir
et al., 2013a, 2013b). The combination of positive assays generates the
list of bacteria possibly present into the sample (presumptive positive)
according to the decision tree presented in Fig. 2.