ROS plays an increasingly important role in apoptosisinduced
cell death (28). Therefore, we determined the effect
of B16 on ROS generation by measuring the fluorescence of
the cell-permeable dye H2DCF-DA in HeLa cells during a 6-h
treatment with 25 µM B16. The mean H2DCF-DA fluorescence
value increased from 49.78 (0 h) to 101.98 (3 h) following treatment
with B16, whereas the value of H2DCF-DA fluorescence
decreased after the 3-h time point (Fig. 6A). These results
indicate that treatment with B16 induced changes in ROS
generation