Severe lesions along with black spots on leaves, inflorescence and fruits on mango (Magnifera indica L.) were observed during routine survey at several sites with mango trees in Eastern Uttar Pradesh indicating the occurrence of anthracnose disease. The pathogen was isolated from several infected samples and matched characteristics of Colletotrichum gloeosporioides Penz. and Sacc. by microscopic, cultural studies and on the basis of spore formation.