Data (Fig. 5) clearly show that (80 °C and 65 °C) were optimal for l-methioninase activity produced by strains No. 4 and No. 60 respectively followed by a slight decrease when higher temperatures were applied. Optimum temperature for l-methioninase produced by several organisms was also reported by many authors. [30] used 25 °C for Brevibacterium linens, [22] used 37 °C on Aspergillus Flavipes and [36] reported that 45 °C was optimum for l-methioninase activity produced by Candida tropicalis, while 37 °C was the optimum temperature for l-methioninase obtained from Pseudomonas putida [22].