Many microorganisms are able to produce enzyme complexes with a good potential for the enzymatic hydrolysis of lignocellulosic materials. Among them, mutant strains of Penicillium echinulatum are known to secrete high levels of filter paper activities (FPA) (Dillon et al., 2011; Reis et al., 2014) and a good ratio between b-glucosidases and FPA when compared to Trichoderma reesei (Martins et al., 2008). Also, these enzymes are very stable at temperatures around 50 C (Camassola et al., 2004).