2.4. Measurements of physiological characteristics
Chlorophyll content was measured at 60 days after germination
with the help of chlorophyll meter (Model SPAD-502, Minolta,
Japan) in three randomly selected flag leaves. Photosynthetic rate
(Pn; mM m2 s1
), transpiration rate (Tr; mM m2 s1
) andstomatal conductance (Gs; Mm2 s1
) were measured on two
consecutive days from two randomly selected flag leaves from
each replication using Infrared Gas Analyzer (IRGA) (LCi-SD, ADC
Bioscientific Ltd., England) at the same stage as chlorophyll
content.