In this study, recombinant malic acid-producing strain 2103a-68 has been further characterized by evaluating the
transcript levels of the overexpressed genes and correlating the results to intracellular activity assays of key metabolic
pathway enzymes.
Furthermore, 13C flux analysis has given new insights into the metabolism of the engineered strain.
The performance of this strain was also evaluated on xylose and a glucose/xylose mixture.