obtained by plotting the percentage disappearance of DPPH as a function of the sample concentration. To 250 lL of DPPH solution, 28 lL of sample mixture was added in a microplate, covered with aluminium foil and incubated for 1 h at room temperature. Absorbance was then read at 517 nm using a UV spectrophotometer microplate reader (Zenyth 200rt Biochrom, UK).