It was
then increased to 175°C at a rate of 4°C/min and was
maintained for 27 min. Finally, the temperature was
increased to 214°C at a rate of 4°C/min and maintained
for 11 min and increased to 225°C at a rate of 4°C/
min and held for 5.5 min; therefore, total run time was
79.25 min