Simultaneous utilization of glucose and xylose by T.
cutaneum
To elucidate the substrate-assimilation profile by T.
cutaneum using sugar mixtures as the carbon source,
the time course of cell performance was determined
(Figure 1). The initial culture was prepared based on a
sugar mass containing 47 g/L glucose and 23 g/L xylose.
However, because the xylose concentration in the mixture
was estimated by subtracting glucose (determined
by the glucose analyzer) from the total sugar (determined
by the DNS method), it appeared to be higher
than expected. The results indicated that the concentrations
of glucose and xylose decreased over time, and
they were 2.6 and 1.1 g/L, respectively, when the cultivation
was stopped after 120 hours. Both sugars were
assimilated simultaneously rather than sequentially. No
diauxic behavior was seen; the cell mass and lipid
increased without any lag period during the whole
process.