Other instrumental conditions were also investigated and a band width of 6 mm and inter-space between bands of 5 mm were found optimal. Scanning of separated bands was carried out at 270 nm using slit dimensions of 6 mm × 0.3 mm where good sensitivity and minimum noise was obtained. Selected slit dimensions enabled scanning of analyte bands without interference from adjacent bands. Various separation parameters; resolution (Rs), peak symmetry (As), capacity factor (Ḱ), separation factor (α) and number of theoretical plates (N) were calculated ( Sherma et al., 1999 and The United States Pharmacopoeia & National Formulary, 2011) in order to evaluate the efficiency of separation. Results showed good resolution and symmetry of the obtained peaks. An excellent separation factor and capacity factor were also demonstrated with a number of theoretical plates approaching those obtained with high performance TLC separations (Table 1).