The lipopeptide showed emulsifying activity against a variety of hydrocarbons and attainedemulsion indices of 81 and 84% with Hexane and cyclohexane, respectively. The lipopeptide showed stability over a temperature range of (25–125◦ C), pH range of (5–12) and salinity range of (5–20%, w/v). The results reveal the potential of the biosurfactant for use in bioremediation of hydrocarbon contaminated environmental media and washing of petroleum sludge contaminated soil