The highest prevalence was found in Nanuque (13.7%)and Belo Horizonte (12.0%) regions where temperatures
were higher. On the other hand, the lowest prevalence was found in Lavras where temperatures were lower than the
other regions (data not shown). The results presented here indicate that canine babesiosis is endemic in rural areas in
the State of Minas Gerais and the only subspecies present is B. canis vogeli. Besides, infection transmission is influenced
by climatic conditions, mainly the temperature.