Cells were pretreated for 2 h with garlic extracts (0.1%) before being stimulated with LPS (1 lg/mL) for 24 h. Media were collected,
and cytokines and nitrite levels were measured by ELISA kit and Griess reagents, respectively. (E) The LPS-induced iNOS upregulation is suppressed by FRGE treatment. Cells
were pretreated for 2 h with FRGE (0.1%) or HRGE (0.1%) before being stimulated with LPS (1 lg/mL) for 24 h.