The size of the inoculum, corresponding to 2% dry matter, is the average number of yeasts recycled by the process of Melle-Boinot applied industrially in the region. It was observed 12% of alcohol production after 10 h of fermentation,
slightly higher to the value currently obtained in the local industry at the same time (10%). Consequently, the scale up from lab scale reactors to 10L bioreactors was successful both in biomass and ethanol production using the yeast S. cerevisiae A2. Therefore, the biomass production of yeast strains isolated naturally in sugary environments is a key point in this sustainable circuit oriented to achieve technological and industrial improvements.