2.4. Phylogenetic analysis
All sequences were compared with ITS sequences closest downloaded from the GenBank database via BLAST search ( Altschul et al., 1990). The sequences were aligned using Clustal X 1.83 with default settings visually (Thompson et al., 1997). The Neighbor-joining (NJ) phylogenetic trees were inferred using heuristic search with 1000 replicates of random sequence addition by using Molecular Evolutionary Genetics Analysis (MEGA 4) (Tamura et al., 2007). Branches corresponding to partitions reproduced in less than 70% bootstrap replicates were collapsed. All positions containing gaps and missing data were eliminated from the dataset (complete deletion option) (Mysore et al., 2011).