Diseased fruit tissues (5 X 3 X 1 mm) from
advancing margins were obtained with a steri-lised scalpel after peeling off the epidermis. Four
pieces of such tissues were embedded in 2 %
water agar or Pimaricin Vancomycin PCNB
media (Ocana and Tsao, 1966). Single
sporangial cultures of two representative isolates
designated PDFD (isolate from Dengkil), and
PDFK (isolate from Kluang) were obtained and
maintained on Vegetable Juice (V8) Agar (VJA)
(Miller, 1955) slants at 24°C and used for the
studies.