Method details
In the water treatment process, traditional CIP techniques can usually remove or sterilize microbes
on the surface of pipes. Taking the advantages of low cost and low energy consumption, these
strategies were universally used in food, fermentation, and water treatment industry [1,3]. However,
when the biofilm forms in pipelines, the traditional methods would not be available to eliminate it
completely [2]. By contrast, the strategy of using muramidase to remove the biofilm in pipes is more
effective and in-depth. The comparison of effectiveness between the traditional CIP and enzyme
cleaning technique is shown in Fig. 1 and Table 1.