Abstract— The probiotic agent designated as Sb
2, isolated from seabass (Lates calcarifer), was identified as Lactococcus lactis subsp. lactis by 16s rDNA. Bacteriocin gene of this strain was identified as Nisin A by PCR technique using specific primer. This strain was applied as starter culture in beef Nham. Survival of L. lactis subsp. lactis Sb 2 during Nham fermentation at
30ºC for 3 days was detected by PCR-RAPD fingerprint technique. The 3 distinct DNA bands about 1200, 2000 and 3000 bp, which corresponded to DNA fingerprint of this strain, were found from day1 to day 3 of fermentation. This confirmed the survival of L. lactiss subsp. lactis Sb 2 during Nham fermentation process.