Serotyping was carried out to determine incriminated
specie for Salmonella using Polyvalent O Antisera. Two
seperate loopful of normal saline was placed on a clean
glass slide after which a clean wire loop was used to take a
small part of suspect colony grown overnight on nutrient
agar and placed on both drops of the normal saline and
thoroughly mixed. A loopful of the antisera was placed on
one of the bacteria suspension while normal saline was
placed on the other (control). Both bacteria suspensions
were mixed using a sterile wire loop and gently tilted to
observe for agglutination. Positive Salmonella spp. with
somatic antigen is indicative of agglutination reaction