Regarding the importance of tumor suppressor genes in the development and progression of cancer, we prompted to understand the potential features which may impact the regulation of these genes in human. In this study, we have correlated the compositional features and expression level of tumor suppressor genes in human tissues. Elucidating any correlation between these factors can lead to a better biological understanding of tumor suppressor genes. Also, the results may help to find some clues about the regulation of these genes in cancer initiation and progression.